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procaspase 3  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc procaspase 3
    Procaspase 3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/procaspase+3/pm41861024-282-0-4
    Average 86 stars, based on 1 article reviews
    procaspase 3 - by Bioz Stars, 2026-09
    86/100 stars

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    Related Articles

    Western Blot:

    Article Title: The protective role of CD44 and microRNA-146a in tendinopathy
    Article Snippet: .. Cell lysates were subjected to immunoblot analyses with antibodies against Smad4 (#ab40759; Abcam, UK), cleaved caspase 3 (c-Cas3, #9664; CST), and procaspase 3 (p-Cas3, #9662; CST), along with a horseradish peroxidase–conjugated secondary antibody (#213110; GeneTex) and quantitative control anti-actin antibodies (#A3854; Sigma-Aldrich). ..

    Control:

    Article Title: The protective role of CD44 and microRNA-146a in tendinopathy
    Article Snippet: .. Cell lysates were subjected to immunoblot analyses with antibodies against Smad4 (#ab40759; Abcam, UK), cleaved caspase 3 (c-Cas3, #9664; CST), and procaspase 3 (p-Cas3, #9662; CST), along with a horseradish peroxidase–conjugated secondary antibody (#213110; GeneTex) and quantitative control anti-actin antibodies (#A3854; Sigma-Aldrich). ..

    Membrane:

    Article Title: Antitumor Activity of Death Receptor 5-Targeted Camptothecin-Loaded Nanoparticles in Murine Syngeneic Models.
    Article Snippet: .. The membrane was probed with anti-FLIP (Cell Signaling Technology #56343, 1:2000), BAX (Cell Signaling Technology #5023, 1:2000), Bcl-2 (Cell Signaling Technology #2764, 1:2000), FADD (BD Biosciences #556402, 1:2000), procaspase-3 (Cell Signaling Technology #9662S, 1:2000), cleaved caspase-3 (Cell Signaling Technology #9661S, 1:1000), PARP (Cell Signaling Technology #9542, 1:2000) or β-actin (Sigma #A5316, 1:10,000) primary antibodies overnight at 4 °C, washed in PBST (3 × 10 min) and subsequently incubated with HRP-conjugated antimouse IgG (Cell Signaling Technology #7076) or antirabbit IgG (Cell Signaling Technology #7074) secondary antibodies for 1 h at room temperature. .. After a further wash in PBST as before, the membrane https://doi.org/10.1021/acs.biomac.5c01884 Biomacromolecules XXXX, XXX, XXX−XXX C was covered in Western Lightning Plus-ECL substrate (PerkinElmer) and imaged using a G:BOX Chemi XX6 gel doc system (Syngene) equipped with GeneSys software.

    Article Title: Antitumor Activity of Death Receptor 5‑Targeted Camptothecin-Loaded Nanoparticles in Murine Syngeneic Models
    Article Snippet: .. The membrane was probed with anti-FLIP (Cell Signaling Technology #56343, 1:2000), BAX (Cell Signaling Technology #5023, 1:2000), Bcl-2 (Cell Signaling Technology #2764, 1:2000), FADD (BD Biosciences #556402, 1:2000), procaspase-3 (Cell Signaling Technology #9662S, 1:2000), cleaved caspase-3 (Cell Signaling Technology #9661S, 1:1000), PARP (Cell Signaling Technology #9542, 1:2000) or β-actin (Sigma #A5316, 1:10,000) primary antibodies overnight at 4 °C, washed in PBST (3 × 10 min) and subsequently incubated with HRP-conjugated antimouse IgG (Cell Signaling Technology #7076) or antirabbit IgG (Cell Signaling Technology #7074) secondary antibodies for 1 h at room temperature. .. After a further wash in PBST as before, the membrane was covered in Western Lightning Plus-ECL substrate (PerkinElmer) and imaged using a G:BOX Chemi XX6 gel doc system (Syngene) equipped with GeneSys software.

    Incubation:

    Article Title: Antitumor Activity of Death Receptor 5-Targeted Camptothecin-Loaded Nanoparticles in Murine Syngeneic Models.
    Article Snippet: .. The membrane was probed with anti-FLIP (Cell Signaling Technology #56343, 1:2000), BAX (Cell Signaling Technology #5023, 1:2000), Bcl-2 (Cell Signaling Technology #2764, 1:2000), FADD (BD Biosciences #556402, 1:2000), procaspase-3 (Cell Signaling Technology #9662S, 1:2000), cleaved caspase-3 (Cell Signaling Technology #9661S, 1:1000), PARP (Cell Signaling Technology #9542, 1:2000) or β-actin (Sigma #A5316, 1:10,000) primary antibodies overnight at 4 °C, washed in PBST (3 × 10 min) and subsequently incubated with HRP-conjugated antimouse IgG (Cell Signaling Technology #7076) or antirabbit IgG (Cell Signaling Technology #7074) secondary antibodies for 1 h at room temperature. .. After a further wash in PBST as before, the membrane https://doi.org/10.1021/acs.biomac.5c01884 Biomacromolecules XXXX, XXX, XXX−XXX C was covered in Western Lightning Plus-ECL substrate (PerkinElmer) and imaged using a G:BOX Chemi XX6 gel doc system (Syngene) equipped with GeneSys software.

    Article Title: Novel 1-(2-Aryl-2-adamantyl)piperazine Derivatives Exhibit In Vitro Anticancer Activity Across Various Human Cancer Cell Lines, with Selective Efficacy Against Melanoma
    Article Snippet: Equal amounts of total protein (25 μg) were resolved on 10 and 12% acrylamide gels and transferred to 0.22 μm pore size polyvinylidene difluoride (PVDF) membranes (Immobilon-PSQ transfer membrane Millipore, Burlington, MA, USA, ISEQ85R). .. Membranes were blocked in blocking buffer for one hour at RT followed by overnight incubation at 4 °C with the following primary antibodies: b-actin (42 kDa; Santa Cruz, Heidelberg, Germany, sc-8432; dilution 1:3000), procaspase 3 (17 kDa; Cell signaling, #14220; dilution 1:1000), LC3 A/B-I/II (14, 16 kDa; Cell signaling, #12741; dilution 1:1000), PARP (116 and 89 kDa; Cell signaling, #9542; dilution 1:1000), and GβL (37 kDa; Cell signaling, #3274; dilution 1:1000). ..

    Article Title: Antitumor Activity of Death Receptor 5‑Targeted Camptothecin-Loaded Nanoparticles in Murine Syngeneic Models
    Article Snippet: .. The membrane was probed with anti-FLIP (Cell Signaling Technology #56343, 1:2000), BAX (Cell Signaling Technology #5023, 1:2000), Bcl-2 (Cell Signaling Technology #2764, 1:2000), FADD (BD Biosciences #556402, 1:2000), procaspase-3 (Cell Signaling Technology #9662S, 1:2000), cleaved caspase-3 (Cell Signaling Technology #9661S, 1:1000), PARP (Cell Signaling Technology #9542, 1:2000) or β-actin (Sigma #A5316, 1:10,000) primary antibodies overnight at 4 °C, washed in PBST (3 × 10 min) and subsequently incubated with HRP-conjugated antimouse IgG (Cell Signaling Technology #7076) or antirabbit IgG (Cell Signaling Technology #7074) secondary antibodies for 1 h at room temperature. .. After a further wash in PBST as before, the membrane was covered in Western Lightning Plus-ECL substrate (PerkinElmer) and imaged using a G:BOX Chemi XX6 gel doc system (Syngene) equipped with GeneSys software.

    Blocking Assay:

    Article Title: Novel 1-(2-Aryl-2-adamantyl)piperazine Derivatives Exhibit In Vitro Anticancer Activity Across Various Human Cancer Cell Lines, with Selective Efficacy Against Melanoma
    Article Snippet: Equal amounts of total protein (25 μg) were resolved on 10 and 12% acrylamide gels and transferred to 0.22 μm pore size polyvinylidene difluoride (PVDF) membranes (Immobilon-PSQ transfer membrane Millipore, Burlington, MA, USA, ISEQ85R). .. Membranes were blocked in blocking buffer for one hour at RT followed by overnight incubation at 4 °C with the following primary antibodies: b-actin (42 kDa; Santa Cruz, Heidelberg, Germany, sc-8432; dilution 1:3000), procaspase 3 (17 kDa; Cell signaling, #14220; dilution 1:1000), LC3 A/B-I/II (14, 16 kDa; Cell signaling, #12741; dilution 1:1000), PARP (116 and 89 kDa; Cell signaling, #9542; dilution 1:1000), and GβL (37 kDa; Cell signaling, #3274; dilution 1:1000). ..



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    Emestrin‐type ETPs inhibited pyroptosis in THP‐1 differentiated macrophages through activation of <t>procaspase‐3/7.</t> (A) Immunoblotting and Coomassie blue staining of cell lysates from THP‐1 differentiated macrophages treated with or without compound 2 (1 µM) 4 h post‐LPS and nigericin induction. P10 band (red rectangle) was sent for mass spectrum analysis. (B) N‑terminal sequence analysis result of p10 band. (C) Mechanism hypothesis of compounds 1 – 11 inhibiting pyroptosis. (D and E) Western blotting analyses of GSDMD, caspase‐3, caspase‐7 cleavage, and α‐tubulin in THP‐1 macrophages treated compound 2 (1 µM) prior and after 4 h pyroptosis induction with LPS and nigericin (mean ± SD; n = 3 per group, t ‐test, ** p < 0.01, **** p < 0.0001). (F) Western blotting analyses of caspase‐1, caspase‐3, caspase‐7 cleavage, and α‐tubulin in the THP‐1 differentiated macrophages treated with each compound (1 µM) 4 h after pyroptosis induction with LPS and nigericin. (G and H) Western blotting analyses of recombinant human procaspase‐3 protein 12 h postincubation with PAC‐1 and each compound (5 µM) respectively at 37 ℃. (I) The IC 50 result of these eleven compounds ( n = 3 per group). (J) In vitro activation of recombinant human procaspase‐3 protein by eleven compounds respectively ( n = 3 per group) and corresponding EC 50 values.
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    Image Search Results


    NF-κB and Caspase-1 activation persists in spleens of SIV-infected RMs despite ART. (a) Representative Western blot analysis of phosphorylated NF-κB p65 (Ser536), total NF-κB p65, cleaved caspase-1 p20 and procaspase-1 in splenic lysates from uninfected control and SIV-infected RMs on ART. (b) Densitometric quantification of the phospho–NF-κB/total NF-κB and caspase-1 p20/procaspase-1 ratio is indicated. Data represent mean ± SEM; * P < 0.05 and ** P < 0.01 by unpaired t test.

    Journal: Frontiers in Immunology

    Article Title: Persistent activation of monocytes/macrophages and cell senescence in SIV-infected macaques on ART

    doi: 10.3389/fimmu.2026.1788994

    Figure Lengend Snippet: NF-κB and Caspase-1 activation persists in spleens of SIV-infected RMs despite ART. (a) Representative Western blot analysis of phosphorylated NF-κB p65 (Ser536), total NF-κB p65, cleaved caspase-1 p20 and procaspase-1 in splenic lysates from uninfected control and SIV-infected RMs on ART. (b) Densitometric quantification of the phospho–NF-κB/total NF-κB and caspase-1 p20/procaspase-1 ratio is indicated. Data represent mean ± SEM; * P < 0.05 and ** P < 0.01 by unpaired t test.

    Article Snippet: Primary antibodies were as follows: rabbit mAb against phospho-NF-κB p65 (Ser536) (93H1) (#3033, Cell Signaling Technology), NF-κB p65 (D14E12) XP ® (#8242, Cell Signaling Technology) and CASPASE 1 p20 (Cleaved Asp296) (PA5-99390, Invitrogen), and mouse mAb antibody against PROCASPASE-1 (D-3) (sc-392736, Santa Cruz Biotechnology).

    Techniques: Activation Assay, Infection, Western Blot, Control

    Emestrin‐type ETPs inhibited pyroptosis in THP‐1 differentiated macrophages through activation of procaspase‐3/7. (A) Immunoblotting and Coomassie blue staining of cell lysates from THP‐1 differentiated macrophages treated with or without compound 2 (1 µM) 4 h post‐LPS and nigericin induction. P10 band (red rectangle) was sent for mass spectrum analysis. (B) N‑terminal sequence analysis result of p10 band. (C) Mechanism hypothesis of compounds 1 – 11 inhibiting pyroptosis. (D and E) Western blotting analyses of GSDMD, caspase‐3, caspase‐7 cleavage, and α‐tubulin in THP‐1 macrophages treated compound 2 (1 µM) prior and after 4 h pyroptosis induction with LPS and nigericin (mean ± SD; n = 3 per group, t ‐test, ** p < 0.01, **** p < 0.0001). (F) Western blotting analyses of caspase‐1, caspase‐3, caspase‐7 cleavage, and α‐tubulin in the THP‐1 differentiated macrophages treated with each compound (1 µM) 4 h after pyroptosis induction with LPS and nigericin. (G and H) Western blotting analyses of recombinant human procaspase‐3 protein 12 h postincubation with PAC‐1 and each compound (5 µM) respectively at 37 ℃. (I) The IC 50 result of these eleven compounds ( n = 3 per group). (J) In vitro activation of recombinant human procaspase‐3 protein by eleven compounds respectively ( n = 3 per group) and corresponding EC 50 values.

    Journal: MedComm

    Article Title: Emestrin‐Type Epidithiodiketopiperazines Inhibited Gasdermin D‐Mediated Pyroptosis via Caspase‐3/7 Activation

    doi: 10.1002/mco2.70548

    Figure Lengend Snippet: Emestrin‐type ETPs inhibited pyroptosis in THP‐1 differentiated macrophages through activation of procaspase‐3/7. (A) Immunoblotting and Coomassie blue staining of cell lysates from THP‐1 differentiated macrophages treated with or without compound 2 (1 µM) 4 h post‐LPS and nigericin induction. P10 band (red rectangle) was sent for mass spectrum analysis. (B) N‑terminal sequence analysis result of p10 band. (C) Mechanism hypothesis of compounds 1 – 11 inhibiting pyroptosis. (D and E) Western blotting analyses of GSDMD, caspase‐3, caspase‐7 cleavage, and α‐tubulin in THP‐1 macrophages treated compound 2 (1 µM) prior and after 4 h pyroptosis induction with LPS and nigericin (mean ± SD; n = 3 per group, t ‐test, ** p < 0.01, **** p < 0.0001). (F) Western blotting analyses of caspase‐1, caspase‐3, caspase‐7 cleavage, and α‐tubulin in the THP‐1 differentiated macrophages treated with each compound (1 µM) 4 h after pyroptosis induction with LPS and nigericin. (G and H) Western blotting analyses of recombinant human procaspase‐3 protein 12 h postincubation with PAC‐1 and each compound (5 µM) respectively at 37 ℃. (I) The IC 50 result of these eleven compounds ( n = 3 per group). (J) In vitro activation of recombinant human procaspase‐3 protein by eleven compounds respectively ( n = 3 per group) and corresponding EC 50 values.

    Article Snippet: Recombinant human procaspase‐3 protein (CUSABIO; Cat# CSB‐EP004548HU) was diluted to 200 ng/μL in activation buffer (20 mM HEPES, pH 7.5, 100 mM NaCl, 1 mM EDTA, 10% glycerol, and 0.1% CHAPS).

    Techniques: Activation Assay, Western Blot, Staining, Sequencing, Recombinant, In Vitro

    Emestrin‐type ETPs directly bound to human procaspase‐3 protein. (A) Molecular docking analyses of compounds 1 and 3 – 11 with human procaspase‐3 protein (PDB ID: 3DEK). (B) Molecular docking analysis of compound 2 with human procaspase‐3 protein. (C) Compound binding activity to recombinant human procaspase‐3 protein measured by SPR. (D and E) SPR test of compound 2 interacting with recombinant human procaspase‐3 protein. The K d value is 76.72 µM. (F and G) Western blotting analyses of recombinant human procaspase‐3 protein different time points after incubation with PAC‐1 (5 µM) and compound 2 (5 µM) (mean ± SD; n = 3 per group, t ‐test, ** p < 0.01). (H and I) CETSA results for compound 2 (5 µM) in THP‐1 differentiated macrophage lysates.

    Journal: MedComm

    Article Title: Emestrin‐Type Epidithiodiketopiperazines Inhibited Gasdermin D‐Mediated Pyroptosis via Caspase‐3/7 Activation

    doi: 10.1002/mco2.70548

    Figure Lengend Snippet: Emestrin‐type ETPs directly bound to human procaspase‐3 protein. (A) Molecular docking analyses of compounds 1 and 3 – 11 with human procaspase‐3 protein (PDB ID: 3DEK). (B) Molecular docking analysis of compound 2 with human procaspase‐3 protein. (C) Compound binding activity to recombinant human procaspase‐3 protein measured by SPR. (D and E) SPR test of compound 2 interacting with recombinant human procaspase‐3 protein. The K d value is 76.72 µM. (F and G) Western blotting analyses of recombinant human procaspase‐3 protein different time points after incubation with PAC‐1 (5 µM) and compound 2 (5 µM) (mean ± SD; n = 3 per group, t ‐test, ** p < 0.01). (H and I) CETSA results for compound 2 (5 µM) in THP‐1 differentiated macrophage lysates.

    Article Snippet: Recombinant human procaspase‐3 protein (CUSABIO; Cat# CSB‐EP004548HU) was diluted to 200 ng/μL in activation buffer (20 mM HEPES, pH 7.5, 100 mM NaCl, 1 mM EDTA, 10% glycerol, and 0.1% CHAPS).

    Techniques: Binding Assay, Activity Assay, Recombinant, Western Blot, Incubation